T2569

Trizma^® hydrochloride solution

pH 7.8, BioPerformance Certified, 1 M, suitable for cell culture

• Synonym(s): Tris hydrochloride solution
• UNSPSC Code: 12161700
• NACRES: NA.25

Properties

• grade: BioPerformance Certified; Molecular Biology
• Quality Level: 200
• form: solution
• concentration: 1 M
• technique(s): cell culture | mammalian: suitable
• impurities: DNase, RNase, Protease, none detected; bioburden, tested; endotoxin, tested; ≤5 ppm Heavy metals (as Pb)
• pH: 7.8
• useful pH range: 7.0-9.0
• absorption: ≤0.05 at 290 at 40%

Description

General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Tris(hydroxymethyl)aminomethane (Tris) is a routine buffer in biological sciences. The pH buffering range is between 7.0 -9.0. It displays negligible binding towards metal in solutions. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. Its low ionic mobility finds usage in capillary electrochromatography as well.

Application

Trizma^® hydrochloride solution has been used:

• as a component of lysis buffer for lysing human embryonic kidney (HEK) 293 cells
• in the 10X digoxigenin (DIG) deoxynucleoside triphosphate (dNTP) nick translation labeling buffer and in tris EDTA and sodium chloride (TEN) buffer
• in the preparation of tris, acetate, ethylenediaminetetraacetic acid (EDTA) (1x TAE) buffer and as a component of 10X gel loading dye

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves