S6639

SSC Buffer 20× Concentrate

for Northern and Southern blotting, solution

• Synonym(s): 20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer
• UNSPSC Code: 41105319
• NACRES: NA.25

Properties

• grade: Molecular Biology
• Quality Level: 200
• description: Saline-Sodium Citrate buffer, made with ultrapure water
• sterility: 0.2 μm filtered
• form: solution
• application(s): diagnostic assay manufacturing
• foreign activity: DNAse, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; RNAse, none detected
• storage temp.: room temp

Description

General description

SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.

Application

SSC Buffer 20× Concentrate has been used

• as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
• as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
• as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon

Features and Benefits

• Convenient ready-to-use liquid
• Multi-purpose use in hybridizations

Preparation Note

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable