Propidium iodide

Name: Propidium iodide
Brand: SIAL

≥94% (HPLC)

Synonym(s):

3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide

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About This Item

Empirical Formula (Hill Notation):

C₂₇H₃₄I₂N₄

CAS Number:

25535-16-4

Molecular Weight:

668.39

Beilstein:

3843838

EC Number:

247-081-0

MDL number:

MFCD00011921

UNSPSC Code:

12352200

PubChem Substance ID:

329769221

NACRES:

NA.77

Key Documents

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Quality Level

200

Assay

≥94% (HPLC)

form

powder

mp

220-225 °C (dec.) (lit.)

fluorescence

λ_ex 482 nm; λ_em 608 nm (pH 7.2)
λ_ex 540 nm; λ_em 608 nm (bound to DNA)

storage temp.

2-8°C

SMILES string

[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

General description

PI is used for selective labelling of dead cells.

Propidium iodide is a fluorescent intercalating agent used for staining. It functions as an apoptosis marker and has linear DNA-binding capacity. Propidium iodide is an effective indicator of tissue injury.

Application

Propidium iodide has been used:

to stain DNA in cell cycle analysis
to stain cells and cell nuclei
for the preparation of propidium iodide (PI)stock solution
to stain cells for flow cytometry

in scrape-loading dye transfer assay

Features and Benefits

This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.