I0408

Invertase-Glykoprotein-Standard

BioReagent, from Saccharomyces cerevisiae

• Synonym(e): Invertase aus Backhefe (S. cerevisiae), β-D-Fructofuranosid-fructohydrolase, β-D-Fructofuranosidase, Saccharase
• CAS Number: 9001-57-4
• EG-Nummer: 3.2.1.26 (BRENDA, IUBMB)
• EG-Nummer: 232-615-7
• MDL-Nummer: MFCD00131410
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: Saccharomyces cerevisiae
• Qualitätsniveau: 200
• Produktlinie: BioReagent
• Form: lyophilized powder
• Mol-Gew.: 60 kDa
• Konzentration: ≥0.5 mg/vial protein (E1%/280)
• Lagertemp.: 2-8°C

Beschreibung

Anwendung

Wird in der Produktion von Süßwaren und Kunsthonig eingesetzt.

Invertase Glycoprotein Standard has been used:

• to generate N-linked glycan library
• as a negative control to study the binding of lectins to high mannose structures
• for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis

The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.

Biochem./physiol. Wirkung

Invertase hydrolysiert Saccharose zu Glucose und Fructose , dieses Reaktionsprodukt ist farblos. Im Gegensatz dazu werden bei der sauren Hydrolyse gefärbte Produkte erhalten.

Sonstige Hinweise

Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)