F8435

PNGase F aus Elizabethkingia meningoseptica

lyophilized powder, recombinant, expressed in E. coli

• Synonym(e): N-Glycosidase F, PNGase F aus Chryseobacterium meningosepticum, PNGase F aus Flavobacterium meningosepticum, Peptid-N-Glycosidase
• CAS Number: 83534-39-8
• EG-Nummer: 3.5.1.52 (BRENDA, IUBMB)
• MDL-Nummer: MFCD18839214
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Konjugat: (N-linked)
• Qualität: Proteomics Grade
• Form: lyophilized powder
• Spezifische Aktivität: ≥1,000 U/mg
• Haltbarkeit: ≥1 weeks at 2‑8 °C (for reconstituted solution); ≥1 yr at -20 °C
• Mol-Gew.: ~36 kDa
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Anwendung

Wird zur Deglykolisierung von Protein verwendet.

Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.

Biochem./physiol. Wirkung

Spaltet ein gesamtes Glykan von einem Glykoprotein ab, vorausgesetzt, die glykolysierte Asparagineinheit ist an ihrem Amino- und Carboxyterminus mit einer Polypeptidkette substituiert.

Verpackung

PNGase F was used for deglycosylation of P-glycoprotein in a study to investigate the dual impact of statins on p-glycoprotein and its effect on doxorubicin cytotoxicity in human neuroblastoma cells. It was used to treat a purified protein, mouse cone ultraviolet (MUV) pigment, before use in quantitative immunoblot analysis in a study It is used to deglycosylate N-linked glycoproteins. Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.

Einheitendefinition

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable