EMS0001

PNGase Fast

recombinant, expressed in E. coli

• Synonym(e): N-Glycosidase F, PNGase F, Peptide N-glycosidase
• UNSPSC-Code: 41131616

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Konjugat: (N-linked)
• Qualität: Proteomics Grade
• Form: ready-to-use solution
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Peptide-N-glycosidase F (PNGase F) belongs to an enzyme family, that are mainly used for the deglycosylation of N-linked glycans.

Anwendung

PNGase Fast may be used to immobilize in order to perform deglycosylation. It may also be used to immobilize onto methacrylate based monolithic support to release the N-linked carbohydrate moieties from glycoproteins.

Biochem./physiol. Wirkung

Peptide-N-glycosidase F (PNGase F) cleaves asparagine-linked high mannose, hybrid and complex oligosaccharides from glycoproteins. It can also deaminate the asparagine to aspartic acid. PNGase Fast enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, to be prepared for downstream chromatography or mass spectrometry analysis. PNGase Fast creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids