11585029001

NBT

crystals, pkg of 5 g

• Synonym(e): Nitrotetrazolium-Blauchlorid, 2,2′-Bis-(4-nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylen)-ditetrazoliumchlorid, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylen)-bis-[2-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchlorid], p-Nitroblau-Tetrazoliumchlorid, p-Nitrotetrazoliumblau, NBT, Nitro BT
• Lineare Formel: C₄₀H₃₀N₁₀O₆ · 2Cl
• CAS Number: 298-83-9
• Molekulargewicht: 817.64
• Beilstein: 4115923
• MDL-Nummer: MFCD00012159
• UNSPSC-Code: 41116100
• PubChem Substanz-ID: 329749491

Eigenschaften

• Beschreibung: 4-Nitro blue tetrazolium chloride, crystals
• Qualitätsniveau: 100
• Form: crystals
• Verpackung: pkg of 5 g
• Hersteller/Markenname: Roche
• Lagertemp.: 2-8°C
• SMILES String: [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
• InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
• InChIKey: FSVCQIDHPKZJSO-UHFFFAOYSA-L

Beschreibung

Allgemeine Beschreibung

NBT is soluble in water, aqueous buffers, and dimethylformamide.

Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology. In immunolabeling technique, 4-Nitro blue tetrazolium (NBT) serves as an oxidant. The hydrolysis of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) by alkaline phosphatase gives rise to leucoindigo, which is then being reduced by NBT to form an insoluble blue BCI. Certain cells reduce NBT, which is a colorless/yellow dye to blue or black formazan crystals. Due to this property, NBT is prefered in redox histochemistry and in biochemical applications. It is formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

Anwendung

• Colony and plaque hybridization
• Immunohistocytochemistry
• In situ hybridization
• Northern blot
• Southern blot
• Western blot

NBT has been used in in situ hybridization.

Angaben zur Herstellung

Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.

Hinweis zur Analyse

Absorption: NBT has its maximum absorption at 260 nm [in water],
and the final product formazan has its maximum absorption at 605 nm [in nitrobenzole].

Performance-controlled in the LDH assay.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H302,H315,H319,H335
• P-Sätze: P261 - P264 - P280 - P304 + P340 + P312 - P337 + P313 - P403 + P233
• Gefahreneinstufungen: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash