69385

Protein Standard Mix 15 - 600 kDa

for size exclusion chromatography

• Código UNSPSC: 85151701
• NACRES: NA.24

Propiedades

• Nivel de calidad: 100
• Formulario: solid
• mol peso: 15-600 kDa
• clases químicas de analitos: amino acids, peptides, proteins
• técnicas: gel permeation chromatography (GPC): suitable
• aplicaciones: clinical; food and beverages; pharmaceutical
• Formato: neat
• temp. de almacenamiento: −20°C

Descripción

Descripción general

The protein standard mix is a calibration standard to test and monitor performance of size exclusion chromatography (SEC) columns. It is a lyophilized mixture of molecular weight markers ranging from 15 kDa to 600 kDa.

Components

Thyroglobulin bovine MW ~ 670 000 Da

γ-globulins from bovine blood MW ~ 150 000 Da

Ovalbumin MW~ 44 300 Da

Ribonuclease A type I-A MW ~ 13 700 Da

p-aminobenzoic acid (pABA) MW ~ 137 Da

Aplicación

This analytical standard is used for the following:

• Evaluation of selectivity and separation efficiency of size exclusion chromatography (SEC) to separate intact proteins by varying flow rate, size of silica particles and pore sizes in the column
• Simultaneous determination of oligomerized and nitrated proteins by size exclusion chromatography-high performance liquid chromatography-diode array detection (SEC-HPLC-DAD)
• Molecular weight separation of proteins by size-exclusion chromatography, formed upon O₃ and NO₂ induced oxidation, nitration, and oligomerization of bovine serum albumin (BSA) as a model protein
• Estimation of molecular masses of two recombinant proteins— TNF fluorescent sensor (BTN-Kat) and fluorescent sensor-inhibitor (ITN-Kat), by size exclusion chromatography (SEC) to evaluate their ability of binding and neutralizing tumor necrosis factor (TNF) in vitro and further serving as imaging labels for non-invasive analysis

Información de seguridad

• Pictogramas: GHS08,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H302 + H312 + H332,H334,H412
• Consejos de prudencia: P261 - P264 - P273 - P280 - P301 + P312 - P302 + P352 + P312
• Clasificaciones de peligro: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable