221198

Formamide

ACS reagent, ≥99.5%

• Synonym(s): Amide C₁, Formic amide
• Linear Formula: HCONH₂
• CAS Number: 75-12-7
• Molecular Weight: 45.04
• Beilstein: 505995
• EC Number: 200-842-0
• MDL number: MFCD00007941
• UNSPSC Code: 12352111
• PubChem Substance ID: 24853220
• Grade: ACS reagent
• Assay: ≥99.5%
• Technique(s): PCR: suitable
• Bp: 210 °C (lit.)
• Vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)

Properties

• grade: ACS reagent
• Quality Level: 200
• vapor density: 1.55 (vs air)
• vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• Assay: ≥99.5%
• form: liquid
• autoignition temp.: 932 °F
• technique(s): PCR: suitable
• color: APHA: ≤10
• refractive index: n20/D 1.447 (lit.)
• pH: 4-10 (20 °C, 200 g/L)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• transition temp: freezing point 2.0-3.0 °C
• density: 1.134 g/mL at 25 °C (lit.)
• SMILES string: NC=O
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N

Description

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

The addition of formamide may enhance the specificity and efficiency of amplification of PCR (polymerase chain reaction). It may be used as an ammonia equivalent for the palladium-catalyzed aminocarbonylation of 1-bromo-3-trifluoromethylbenzene to form 3-(trifluoromethyl)benzamide.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H351,H360D,H373
• Precautionary Statements: P201 - P202 - P260 - P280 - P308 + P313 - P405
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• Target Organs: Blood
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): 305.6 °F
• Flash Point(C): 152 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves