E7885

Elastase from porcine pancreas

lyophilized powder, suitable for cell culture

• Sinónimos: Elastase from hog pancreas, Pancreatopeptidase E
• CAS Number: 39445-21-1
• Número CE: 3.4.21.36 (BRENDA, IUBMB)
• Número CE: 254-453-6
• Número MDL: MFCD00130998
• Código UNSPSC: 12352204
• NACRES: NA.75

Propiedades

• origen biológico: Porcine
• Nivel de calidad: 200
• Formulario: lyophilized powder
• actividad específica: ≥4 units/mg protein
• mol peso: 25.9 kDa
• envase: pkg of 1 μmol
• técnicas: cell culture | mammalian: suitable
• pH: 8
• idoneidad: suitable for
• actividad extraña: Trypsin ≤50 BAEE units/mg protein
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

Aplicación

Elastase from porcine pancreas is used for the following applications:

• Cell culture techniques
• ELISA techniques
• Used in assay for elastase inhibitory activity

Acciones bioquímicas o fisiológicas

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Envase

Package size based on protein content

Definición de unidad

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Información de seguridad

• Pictogramas: GHS08,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H315,H319,H334,H335
• Consejos de prudencia: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable