SRE0026

Phosphatase substrate

Suitable for manufacturing of diagnostic kits and reagents

• Synonym(s): Phosphatase substrate, 4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate
• Linear Formula: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• CAS Number: 333338-18-4
• Molecular Weight: 371.14
• EC Number: 224-246-5
• MDL number: MFCD00066288
• UNSPSC Code: 12352204
• PubChem Substance ID: 329826114
• NACRES: NA.32

Properties

• Quality Level: 400
• Assay: ≥97% (HPLC)
• form: powder
• solubility: water: 100 mg/mL, clear to slightly hazy
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C
• SMILES string: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• InChI key: KAKKHKRHCKCAGH-UHFFFAOYSA-L

Description

Application

Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.

Other Notes

Substrate for the determination of acid and alkaline phosphatases.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable