R6148

RNase A solution

Name: RNase A solution
Brand: SIGMA

Synonym(s):

GenElute^™ RNAse A

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.52

Key Documents

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biological source

bovine pancreas

Quality Level

200

form

liquid

specific activity

1500 units/mL
3000 units/mL

concentration

20 mg/mL in Tris buffer

technique(s)

DNA purification: suitable

storage temp.

room temp

Gene Information

bovine ... RNASE1(282340), RNASE1(280930)

General description

Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute^™ kits.

Application

RNase A solution has been used:

in apoptosis assay of Hep-2 cells
for the colorimetric quantification of methylated DNA
for DNA and RNA extraction
in DNA laddering analysis
in cell cycle analysis
as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry

Biochem/physiol Actions

Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.