L8275
Lectin from Galanthus nivalis (snowdrop)
lyophilized powder
Synonym(s):
Galanthus nivalis lectin, GNL
form
lyophilized powder
Quality Level
potency
<20 μg per mL agglutination activity
composition
Protein, ≥90% Lowry
impurities
salt, essentially free
storage temp.
2-8°C
General description
Lectin from Galanthus nivalis (snowdrop) is a tetrameric, 12 kDa mannose binding lectin. It belongs to the family, Amaryllidacea. It is specific to α-1,3- or 1,6- linked D-mannose of carbohydrates. Snowdrop lectins are toxic to insects.
Application
Lectin from Galanthus nivalis (snowdrop) has been used:
- to coat ELISA plates
- as reverse transcriptase inhibitor
- in ELISA for detection of envelope glycoproteins of the human and simian immunodeficiency viruses
Biochem/physiol Actions
GNL agglutinates rabbit erythrocytes; human erythrocytes are not agglutinated. GNL sugar specificity is directed to the nonreducing end of the terminal α-D-mannosyl residue of glycoconjugates.
Lectin from Galanthus nivalis (snowdrop) provide resistance against pests and are used in transgenic plants. Snowdrop lectin functions as a carrier to transport fused proteins to the hemolymph as it crosses gut epithelium of target species to exhibit insecticidal effects.
Preparation Note
Purified by affinity chromatography according to Kaku.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilution in phosphate buffered saline, pH 7.2 of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of rabbit erythrocytes after 1 hr incubation at 25 °C.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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