C6745

Human Collagen Type IV

from human cell culture, liquid, 0.3 mg/mL, suitable for cell culture

• CAS Number: 9007-34-5
• EC Number: 232-697-4
• MDL number: MFCD00130825
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• Product Name: Collagen Type IV from human cell culture, Bornstein and Traub Type IV, 0.3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture
• biological source: human cell culture
• Quality Level: 200
• sterility: sterile-filtered
• product line: BioReagent
• form: solution
• mol wt: 170—180 kDa
• packaging: pkg of 1 mL
• concentration: 0.3 mg/mL
• technique(s): cell culture | mammalian: suitable
• surface coverage: 6‑10 μg/cm²
• impurities: HBSAG, none detected; HCV, none detected; HIV-1/HIV-2, none detected
• NCBI accession no.: NM_001845.6
• UniProt accession no.: P02462
• Binding Specificity: Peptide Source: Laminin; ; Peptide Source: Nidogen
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... COL4A1(1282)

Description

General description

COL4A1 (collagen type IV α 1 chain) belongs to the collagen family. It has an N-terminal 7S domain, a C-terminal globular domain (NC1), and the central triple helical part with short interruptions of the Gly-X-Y repeats . It is an important constituent of type IV collagen in the basement membrane . The COL4A1 gene is located on human chromosome 13q34 .

Collagen IV, derived from human fibroblasts and epithelial cells in a co-culture system, creates an in vitro ECM (extracellular matrix) and serves as an excellent biological scaffold for three dimensional cell culture.

Collagen IV is a heterotrimeric molecules containing two α1-like and one α2-like chain. It is considered essential for completion of embryogenesis and is necessary for proper tissue organization and structural integrity. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. Produced by human fibroblasts and epithelial cells, Collagen IV is then purified biochemically and provided as a sterile solution at a concentration of 0.3mg/ml in 25% acetic acid.

Application

Collagen Type IV from human cell culture has been used in iPSC (induced pluripotent stem cells) culture and differentiation. It has also been used as membrane coatings.

Biochem/physiol Actions

COL4A1 (collagen type IV α 1 chain) related disorder is associated with pulmonary complication. This gene is a major constituent required in basal membrane stability. Mutations in COL4A1 result in familial porencephaly, infantile hemiplegia, cerebral small vessel disease (CSVD) and hemorrhagic stroke.

Features and Benefits

• This Collagen Type IV product is generated by human fibroblasts and epithelial cells in a co-culture system, which produces an ECM in vitro.
• The Collagen Type IV is then purified biochemically.
• It is sterilized by dialysis in an acetic acid and 0.5% chloroform solution and is supplied sterile at a concentration of ~0.3 mg/ml in 25% acetic acid.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves