Accéder au contenu
MilliporeSigma

Determination of the Juglone Content of Juglans regia Leaves by GC/MS.

Natural product communications (2015-09-29)
Irena Matławska, Wiesława Bylka, Ewa Widy-Tyszkiewicz, Beata Stanisz
RÉSUMÉ

The constituents of walnut (Juglans regia L.) leaves are represented by tannins, phenolics, and naphthoquinones, the characteristic compound being juglone. The content of juglone in the methanolic extract of the leaves determined by the GC/MS method was 9.9 ± 0.2 mg/100 g; small amounts (1.3 ± 0.02 mg/100 g) were recorded in the infusion, whereas in the decoction it was not detected. As some studies indicate toxicity of juglone, only decoctions should be recommended for therapeutic use.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Hydroxyde de sodium solution, BioUltra, 10 M in H2O
Sigma-Aldrich
Acide chlorhydrique solution, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
Hydroxyde de sodium solution, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
Acide chlorhydrique, BioReagent, 36.5-38.0%
Supelco
Acide chlorhydrique solution, volumetric, 0.1 M HCl (0.1N), endotoxin free
Sigma-Aldrich
Hydrogen chloride, ReagentPlus®, ≥99%
Sigma-Aldrich
Acide chlorhydrique solution, ~6 M in H2O, for amino acid analysis
Sigma-Aldrich
Hydroxyde de sodium, BioUltra, ≥98.0% (T), pellets
Sigma-Aldrich
Toluène, anhydrous, 99.8%
Sigma-Aldrich
Chlorure d′hydrogène solution, 3 M in cyclopentyl methyl ether (CPME)
Sigma-Aldrich
Méthanol, anhydrous, 99.8%
Sigma-Aldrich
Acide chlorhydrique solution, 32 wt. % in H2O, FCC
Sigma-Aldrich
Hydroxyde de sodium, ultra dry, powder or crystals, 99.99% trace metals basis
Sigma-Aldrich
5-Hydroxy-1,4-naphthoquinone, 97%
Sigma-Aldrich
Chloroforme, ≥99%, PCR Reagent, contains amylenes as stabilizer
Sigma-Aldrich
Formiate d′éthyle, ≥97%, FCC, FG
Sigma-Aldrich
Chloroforme, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
Formiate d′éthyle, reagent grade, 97%
Sigma-Aldrich
Chloroforme, anhydrous, contains amylenes as stabilizer, ≥99%
Sigma-Aldrich
Méthanol, suitable for NMR (reference standard)
Sigma-Aldrich
Formiate d′éthyle, natural, ≥95%, FG
Sigma-Aldrich
Sodium hydroxide-16O solution, 20 wt. % in H216O, 99.9 atom % 16O
Sigma-Aldrich
Methanol solution, suitable for NMR (reference standard), 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
Sigma-Aldrich
3-Ethyl-2,4-pentanedione, mixture of tautomers, 98%
Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
Supelco
Chloroforme, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
Chloroforme, ACS reagent, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%
Sigma-Aldrich
Méthanol, HPLC Plus, ≥99.9%, poly-coated bottles
Sigma-Aldrich
Toluène, suitable for HPLC, ≥99.9%