ZRB2213

Anti-p-Histone H2A(Ser129) Antibody, clone 2M22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): H2A1, HTA1, Histone H2A.1, SPT11
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2M22, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 14 kDa; observed mol wt ~16 kDa
• purified by: using Protein A
• species reactivity: human, yeast
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunocytochemistry: suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_010511
• UniProt accession no.: P04911
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: yeast ... HTA1(851811)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2M22 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Histone H2A phosphorylated on Serine 129.

Immunogen

KLH-conjugated linear peptide corresponding to 10 amino acids from the C-terminal region of yeast Histone H2A.1.

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from HeLa cells treated with UV.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Histone H2A (Ser129) in lysate from HeLa cells treated with UV.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected p-Histone H2A in Yeast Extract.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected p-Histone H2A in HeLa cells serum-starved overnight -/+ Etoposide 20ug/mL for 3 hours.

Peptide Inhibition Assay: Target band detection in UV treated HeLa cells was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

A 1:1,000 dilution from a representative lot detected Histone H2A in HeLa treated with UV.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Histone H2A.1 (UniProt: P04911; also known as H2A) is encoded by the HTA1 (also known as H2A1, SPT11, YD9934.10, YDR225W) gene (Gene ID: 851811) in Saccharomyces cerevisiae (yeast). Histone H2A is one of the 5 main histones that are involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histones are modified post-translationally by acetylation, phosphorylation, methylation, and ubiquitination and these modifications regulate DNA transcription, repair, recombination, and replication. Histone H2A.1 undergoes phosphorylation at Serine 129 that plays a crucial role in various cellular processes involved in DNA repair, genomic stability, and cell cycle regulation. Yeast histone H2A1 contains a [ST]-Q motif (aa 129-130) in the C-terminal region that is phosphorylated by the Mec1 and Tel1 kinases in response to DNA double strand breaks or DNA damage signals. It serves as a recognition mark for the recruitment of DNA repair factors and facilitates the repair of damaged DNA. Overall, Histone H2A.1 phosphorylation at serine 129 is a significant event in maintaining genomic integrity and cell homeostasis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable