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MilliporeSigma

SRP5196

Sigma-Aldrich

I?Bβ , GST tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

NFKBIB, TRIP9

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
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biological source

human

recombinant

expressed in E. coli

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

~56 kDa

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... NFKBIB(4793)

General description

IκBβ is part of the NFκB complex and it inactivates NFκB complex by binding to it and trapping it in the cytoplasm. Phosphorylation of serine residues on IκBβ mediated by IκB kinases leads to its destruction via the ubiquitination pathway thereby allowing activation of the NFκB complex which translocates into the nucleus and binds DNA at kappa-B-binding motifs. The PEST domain of IkBβ can interact with two proteins KBRAS1 and KBRAS2 and this interaction can decrease the rate of degradation IκBβ. IκBβ has been shown to participate in multiple signaling pathways including adipocytokine signaling pathway, B-cell receptor signaling pathway, T-cell receptor signaling pathway and Hypoxia.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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