MAK569

Hydroxyproline Assay Kit

sufficient for 100 colorimetric tests

• Synonym(s): Hydroxyproline Quantification Kit
• UNSPSC Code: 41116104

Properties

• usage: sufficient for 100 colorimetric tests
• Quality Level: 100
• application(s): cosmetics
• detection method: colorimetric
• relevant disease(s): cardiovascular diseases; rheumatological diseases; neurological disorders; cancer; dermatological diseases
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Hydroxyproline, known chemically as 4-hydroxyproline, emerges through the enzymatic modification of proline after protein synthesis, a process termed post-translational hydroxylation. This modified amino acid is predominantly found within collagen, contributing significantly to its triple-helical structure and overall stability. The abundance of hydroxyproline confined almost exclusively to collagen renders it a reliable biomarker for assessing collagen abundance in biological tissues.

Elevations in hydroxyproline can be indicative of heightened collagen metabolism, often observed in conditions that prompt increased collagen synthesis or breakdown. This is particularly evident in scenarios of enhanced bone turnover, where hydroxyproline measurements in bodily fluids can serve as clinical biomarkers for bone resorption and overall bone health.

Collagen′s integral role in the extracellular matrix of connective tissues lends it the tensile strength essential for tissue integrity. In the cosmetic industry, the supplementation of collagen is believed to enhance skin firmness and combat signs of aging, due to the structural support provided by hydroxyproline-containing collagen fibers.

Application

The Hydroxyproline Assay Kit may be used for:

• Cancer Research
• Cardiovascular Research
• Cosmetic Testing
• Dermatological Disease Research

Features and Benefits

Compatibility with High-Throughput Systems: Seamlessly integrate our kit into high-throughput handling systems, ensuring smooth operations and accurate results, enhancing efficiency in your laboratory workflow.


Convenient Data Analysis: Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file

Suitability

Suitable for measuring Hydroxyproline in various biological samples such as cells, tissues, serum, and urine.

Principle

The Hydroxyproline Assay Kit facilitates the measurement of hydroxyproline levels through a reaction where the oxidized form of hydroxyproline interacts with 4-(Dimethylamino)benzaldehyde (DMAB). This interaction produces a colorimetric response that can be measured at a wavelength of 560 nm, with the intensity of the color directly reflecting the concentration of hydroxyproline in the sample.

Safety Information

• Pictograms: GHS02,GHS03,GHS08,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H272,H290,H314,H317,H334,H336,H373
• Precautionary Statements: P210 - P220 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Ox. Liq. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT RE 2 - STOT SE 3
• Target Organs: Central nervous system, Thyroid
• Supplementary Hazards: EUH031
• Storage Class Code: 5.1B - Oxidizing hazardous materials
• WGK: WGK 2
• Flash Point(F): 53.6 °F - closed cup
• Flash Point(C): 12.0 °C - closed cup