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MAK566

Sigma-Aldrich

Acetyl Coenzyme A Assay Kit

sufficient for 100 fluorometric tests

Synonym(s):

Acetyl-CoA Assay Kit

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About This Item

Quality Level

100

detection method

fluorometric

relevant disease(s)

neurological disorders; cancer

storage temp.

−20°C

Related Categories

General description

Acetyl-CoA (AcCoA) is an essential cofactor and carrier of acyl groups in enzymatic acetyl transfer reactions. It is the starting compound for the citric acid cycle (Kreb′s cycle). It is also a key precursor in lipid biosynthesis, and the source of all fatty acid carbons. AcCoA acts as an indicator of fat, sugar, and protein levels and shows up on the nutritional status. In lowered glucose conditions, AcCoA participates in the production of ATP. Increased AcCoA levels are observed in prostate cancer due to elevated fatty acid utilization and thus provides an additional energy source for the tumor cell growth.

Application

The Acetyl Coenzyme A Assay Kit may be used for:
Cancer Metabolism Research
Mitochondrial Metabolism Research
Neurological Research

Features and Benefits

Enhanced Sensitivity and Accuracy: Detect Acetyl-CoA (AcCoA) concentrations ranging from 20 - 1000 pmol providing precise results across a wide range of sample types.

Compatibility with High-Throughput Systems: Seamlessly integrate our kit into high-throughput handling systems, ensuring smooth operations and accurate results, enhancing efficiency in your laboratory workflow.

Convenient Data Analysis: Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file.

Suitability

Suitable for measuring Acetyl-CoA in various biological samples such as cell lysates and tissues.

Principle

Acetyl-CoA concentration is determined by a coupled enzyme assay, which results in a fluorometric (λex = 340/λem = 460 nm) product, proportional to the Acetyl-CoA present. Typical sensitivities of detection for this kit are 20-1000 pmol of Acetyl CoA. This kit is a highly sensitive assay for determining Acetyl-CoA level in a variety of biological samples.

Signal Word

Danger

Hazard Statements

H300,H311,H314,H317,H334

Hazard Classifications

Acute Tox. 2 Oral - Acute Tox. 3 Dermal - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

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Certificates of Analysis (COA)

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