MAK492
Fumarate Assay Kit
sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12352200
Recommended Products
Quality Level
application(s)
food and beverages
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Fumarate, or fumaric acid, is one of the key components in the TCA cycle and is used by cells to form ATP. Human skin, when exposed to sunlight, will naturally produce fumaric acid. Fumarate is used as an additive by the food and beverage industry. Fumaric acid esters are also used to treat psoriasis. Increased urinary fumarate may be due to impaired Krebs Cycle function, a defect in the enzyme fumarase, or mitochondrial function.
Features and Benefits
- Linear detection range 0.005 to 2 mM fumarate.
- Compatible with high-throughput handling systems.
Suitability
The kit is suitable for the determination of fumarate in food, beverage, and other biological samples (e.g. cell lysate, tissue homogenate, and serum).
Principle
The Fumarate Assay Kit is based on the fumarase catalyzed hydration of fumarate to malate. The malate is then oxidized by malate dehydrogenase generating NADH, which reduces a formazan (MTT) dye. The intensity of the product color, measured at 565 nm, is proportional to the fumarate concentration in the sample.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
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