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antibody form
purified antibody
clone
3E6
monoclonal
purified by
using protein G
species reactivity
rat, monkey, mouse, human, mouse, mouse, human
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
UniProt accession no.
General description
GST-tagged Full-length recombinant human Nucleolysin TIAR protein.
Specificity
Clone 3E6 is a mouse monoclonal antibody that detects Nucleolysin TIAR protein. It also displays weak reactivity with TIA-1.
Application
Immunocytochemistry Analysis: A representative lot detected TIAR in Immunocytochemistry applications (Taupin, J.L., et. al. (1995). Proc Natl Acad Sci USA. 92(5):1629-33; Kedersha, N.L., et. al. (1999). J Cell Biol. 147(7):1431-42).
ELISA Analysis: A representative lot detected TIAR in ELISA applications (Taupin, J.L., et. al. (1995). Proc Natl Acad Sci USA. 92(5):1629-33).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected TIAR in Immunohistochemistry applications (Beck, A.R., et. al. (1998). Proc Natl Acad Sci USA. 95(5):2331-6).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TIAR in Immunoprecipitation applications (Taupin, J.L., et. al. (1995). Proc Natl Acad Sci USA. 92(5):1629-33).
Western Blotting Analysis: A representative lot detected TIAR in Western Blotting applications (Beck, A.R., et. al. (1196). Nucleic Acids Res. 24(19):3829-35).
ELISA Analysis: A representative lot detected TIAR in ELISA applications (Taupin, J.L., et. al. (1995). Proc Natl Acad Sci USA. 92(5):1629-33).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected TIAR in Immunohistochemistry applications (Beck, A.R., et. al. (1998). Proc Natl Acad Sci USA. 95(5):2331-6).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TIAR in Immunoprecipitation applications (Taupin, J.L., et. al. (1995). Proc Natl Acad Sci USA. 92(5):1629-33).
Western Blotting Analysis: A representative lot detected TIAR in Western Blotting applications (Beck, A.R., et. al. (1196). Nucleic Acids Res. 24(19):3829-35).
Quality
Evaluated by Western Blotting in human testis tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TIAR in human testis tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TIAR in human testis tissue lysate.
Physical properties
~42 kDa observed; 41.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Human, Monkey, Mouse.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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