HADP-61HK

Human Adiponectin RIA

for protein sequence analysis, for protein analysis

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• grade: for protein analysis
• Quality Level: 100
• description: ED₈₀ = 3.9 ± 0.9 ng/mL <br />ED₅₀ = 21.0 ± 5.7 ng/mL <br />ED₂₀ = 128.0 ± 48.6 ng/mL ; analytes available: Adiponectin; 0.78-200 ng/mL (standard curve range)
• feature: accuracy 101% serum sample concentration 1:500 dilution–100 ng/mL standard; accuracy 111% serum sample concentration 1:500 dilution–20 ng/mL standard; accuracy 116% serum sample concentration 1:500 dilution–5 ng/mL standard
• species reactivity: human
• sensitivity measuring range: 0.78 ng/mL
• technique(s): radioimmunoassay: suitable
• UniProt accession no.: Q15848
• detection method: radioactive

Description

General description

FUNCTION: SwissProt: Q15848 # Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
SIZE: 244 amino acids; 26414 Da
SUBUNIT: Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionnally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Synthesized exclusively by adipocytes and secreted into plasma.DOMAIN:SwissProt: Q15848 The C1q domain is commonly called the globular domain.
PTM: Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. & HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin- sensitizing activity of adiponectin in hepatocytes (By similarity). & O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. & Not N-glycosylated.
DISEASE: SwissProt: Q15848 # Defects in ADIPOQ are the cause of adiponectin deficiency [MIM:605441]. The result is a very low concentration of plasma adiponectin. Decreased adiponectin plasma levels are associated with obesity insulin resistance, and diabetes type 2.
SIMILARITY: Contains 1 C1q domain. & Contains 1 collagen-like domain.
MISCELLANEOUS: Variants Arg-84 and Ser-90 show impaired formation of HMW complexes whereas variants Cys-112 and Thr-164 show impaired secretion of adiponectin in any form. & HMW-complex blood contents are higher in females than in males, are increased in males by castration and decreased again upon subsequent testosterone treatment, which blocks HMW- complex secretion (By similarity). In type 2 diabetic patients, both the ratios of HMW to total adiponectin and the degree of adiponectin glycosylation are significantly decreased as compared with healthy controls.

Inter-assay: 6.9 – 9.3 (serum sample concentration 1:500 dilution – 3 ng/mL, 6 ng/mL, 15 ng/mL) Intra-assay: 1.8 – 6.2 (serum sample concentration 1:500 dilution – 3 ng/mL, 6 ng/mL, 15 ng/mL)

Specificity

Human Adiponectin 100%
C1q Human < 0.01%
Mouse Adiponectin 400%

Application

Overnight incubation at room temperature. <br />Sample volume <2 μL serum or plasma, or <100 μL tissue culture media

Analysis Note

Linearity 85–119%

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H301,H310 + H330,H373,H411
• Precautionary Statements: P262 - P273 - P280 - P302 + P352 + P310 - P304 + P340 + P310 - P314
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - STOT RE 2 Oral
• Target Organs: Brain
• Supplementary Hazards: EUH032
• Storage Class Code: 7 - Radioactive Substances