ECM335-M

Human sICAM-1 ELISA

• UNSPSC Code: 12161503
• eCl@ss: 32161000

Properties

• description: analytes available: sICAM-1
• species reactivity: human
• technique(s): ELISA: suitable
• UniProt accession no.: P05362

Description

General description

FUNCTION: SwissProt: P05362 # ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). In case of rhinovirus infection acts as a cellular receptor for the virus. Also acts as receptor for some coxsackieviruses.
SIZE: 532 amino acids; 57825 Da
SUBUNIT: Interacts with human herpesvirus 8 MIR2 protein (Probable).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
SIMILARITY: SwissProt: P05362 ## Belongs to the immunoglobulin superfamily. ICAM family. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Components

· 1 aluminum pouch with MicroWell^™ plate coated with Monoclonal Antibody (murine) to human ICAM-1 · 1 vial (0.1 mL) HRP-Conjugated Anti-ICAM-1 Monoclonal (murine) Antibody · 2 vials (0.5 mL each) 100 ng/mL soluble ICAM-1 Standard 1 vial lyophilized control (high). 1 vial lyophilized control (low). · 1 bottle (50 mL) Wash Buffer Concentrate 20X (PBS with 1% TWEEN^® 20) · 1 vial (5 mL) Assay Buffer Concentrate 20X (PBS with 2% TWEEN^® 20 and 10% BSA) · 1 bottle (12 mL) Sample Diluent (buffered protein matrix) · 1 vial (15 mL) Substrate Solution · 1 vial (15 mL) Stop Solution (1M Phosphoric Acid) · 1 MicroWell^™ Strip Holder · 2 Adhesive Plate Covers

Storage and Stability

Store kit reagents, except the control, between 2° and 8°C. Store the lyophilized control at -20°C. Immediately after use remaining reagents should be returned to cold storage (2° to 8°C) with the exception of reconstituted control (-20°C). Expiration date of the kit is stated on outside label. Precautions: · Reagents are intended for research use only and are not for use in diagnostic or therapeutic procedures. · Do not mix or substitute reagents with those from other lots or other sources. · Do not use kit reagents beyond expiration date on label. · Do not expose kit reagents to strong light during storage or incubation. · Rubber or disposable latex gloves should be worn while handling kit reagents or specimens. · Some reagents contain thimerosal as preservative, which is highly toxic by inhalation, ingestion, or contact with skin. Thimerosal is a possible mutagen and should be handled accordingly. · Avoid contact of substrate solutions with oxidizing agents and metal. · In order to avoid microbial contamination or cross-contamination of reagents or specimens which may invalidate the test use disposable pipette tips and/or pipettes. · Use clean, dedicated reagent trays for dispensing the conjugate and substrate reagents. · Exposure to acids will inactivate the conjugate. · Glass-distilled water or deionized water must be used for reagent preparation. · Substrate solutions must be at room temperature prior to use. · Since exact conditions may vary from assay to assay, a standard curve must be established for every run. · Bacterial or fungal contamination of either screen samples or reagents or cross-contamination between reagents may cause erroneous results. · Disposable pipette tips, flasks or glassware are preferred. Reusable glassware must be washed and thoroughly rinsed of all detergents before use. · Improper or insufficient washing at any stage of the procedure will result in either false positive or false negative results. Completely empty wells before dispensing fresh Wash Buffer, fill with Wash Buffer as indicated for each wash cycle and do not allow wells to sit uncovered or dry for extended periods. · The use of radioimmunotherapy has significantly increased the number of patients with human anti-mouse IgG antibody (HAMA). HAMA may interfere with assays utilizing murine monoclonal antibodies leading to both false positive and false negative results. HAMA interference can be reduced by adding murine immunoglobulins (serum, ascitic fluid, or monoclonal antibodies of irrelevant specificity) to the Sample Diluent.

Legal Information

MicroWell is a trademark of Thermo Fisher Scientific or its subsidiaries

TWEEN is a registered trademark of Croda International PLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.