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Key Documents

AB3424

Sigma-Aldrich

Anti-Surfactant Protein A Antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

antibody form

serum

Quality Level

clone

polyclonal

description

Target: Pulmonary airways, Eustachian tube epithelia, Type II alveolar cells and Clara cells

species reactivity

sheep, rabbit, bovine

technique(s)

ELISA: suitable
western blot: suitable

UniProt accession no.

General description

FUNCTION: SwissProt: Q8IWL2 # In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.
SIZE: 248 amino acids; 26242 Da
SUBUNIT: Oligomeric complex of 6 set of homotrimers.
SUBCELLULAR LOCATION: Secreted, extracellular space.
DISEASE: SwissProt: Q8IWL2 # Genetic variations in SFTPA1 are associated with respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS in the newborn is the main cause of mortality and morbidity in premature infants. RDS is characterized by deficient gas exchange that is caused by diffuse atelectasis and high-permeability lung edema that results in fibrin-rich alveolar deposits called ′hyaline membranes′. The risk of bronchopulmonary dysplasia increases with the severity of RDS.
SIMILARITY: SwissProt: Q8IWL2 ## Contains 1 C-type lectin domain. & Contains 1 collagen-like domain.
MISCELLANEOUS: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).
Bovine SP-A

Specificity

Reacts strongly with bovine and sheep SP-A.

Application

Immunoblot: 1:1,000-1:5,000

ELISA: 1:1,000-1:30,000

Optimal dilutions must be determined by the end user.

Physical form

Rabbit serum. Liquid. Contains no preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids


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