524733

Phosphodiesterase 4D3, His•Tag^®, Human, Recombinant, S. frugiperda

• Synonym(s): PDE4D3, cAMP-specific phosphodiesterase 4D isoform 3
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• recombinant: expressed in insect cells (S. frugiperda)
• Quality Level: 100
• form: liquid
• specific activity: ≥0.25 U/μL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Full-length, recombinant, human phosphodiesterase 4D3 (accession no. L20970) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole cAMP to AMP per min at pH 7.5, 30°C.

Physical form

In 150 mM NaCl, 10 mM Tris, pH 8.3 .

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Xu, R.X., et al. 2000. Science288, 1822.
Nemoz, G., et al. 1996. FEBS Lett.384, 97.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable