401251

IM-412

IM-412 primarily used in Inhibition.

• Synonym(s): IM-412, 3-(2-chlorobenzyl)-1,7-dimethyl-1H-imidazo[2,1-f]purine-2,4(3H,8H)-dione, TGF beta Inhibitor, IM-412
• Empirical Formula (Hill Notation): C₁₆H₁₄ClN₅O₂
• Molecular Weight: 343.77
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• Quality Level: 100
• Assay: ≥90% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: off-white
• solubility: DMSO: 3 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A cell permeable imidazoxanthine shown to decrease TGF-β stimulated reporter activity in a dose-dependent manner in HEK293 reporter cells, with an IC₅₀ of 4.1 µM without cell cytotoxicity. Inhibits TGF-β receptor I (TβRI) and TGF-β receptor II (TβRII) expression and its downstream Smad2 and Smad3 phosphorylation as well as JNK and ERK activity. In addition, it elicits TGF-β-induced fibroblast differentiation with attenuated expression of several fibrotic markers such as α-SMA and fibronectin, and suppresses collagen accumulation in CCD-18Lu human normal lung fibroblasts.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Park, S., et al. 2010. Biochem. Biophy. Res. Comm.399, 268.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable