T7527

Tris-Borate-EDTA buffer

BioReagent, Molecular Biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

• Sinônimo(s): TBE buffer
• Número MDL: MFCD00236358
• Código UNSPSC: 41105319
• ID de substância PubChem: 329826869
• NACRES: NA.25

Propriedades

• grau: Molecular Biology
• Nível de qualidade: 200
• linha de produto: BioReagent
• Formulário: powder blend
• Impurezas: DNase and RNase, none detected
• pH: 8.1-8.5 (5 ×)
• solubilidade: water: 85.1 g/L, clear, colorless
• adequação: suitable for electrophoresis
• aplicação(ões): diagnostic assay manufacturing
• cadeia de caracteres SMILES: OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
• chave InChI: OSBLTNPMIGYQGY-UHFFFAOYSA-N

Descrição

Aplicação

Ready for use in gel electrophoresis after dilution to working concentrations.

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Embalagem

1L poly bottle contains a powder blend that can be dissolved and reconstituted within the bottle to prepare one liter of a 5× concentrate.
4L poly bottle contains a powder blend that can be dissolved and reconstituted to prepare four liters of a 5× concentrate.

Nota de preparo

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Reconstituição

Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H360FD
• Declarações de precaução: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classificações de perigo: Repr. 1B
• Código de classe de armazenamento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type P2 (EN 143) respirator cartridges