SEQR

SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

• Sinônimo(s): WGA kit, Whole transcriptome amplification
• Código UNSPSC: 41121800
• NACRES: NA.55

Propriedades

• Nível de qualidade: 200
• técnica(s): whole genome amplification: suitable
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

The SeqPlex^™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Aplicação

SeqPlex^™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.

Características e benefícios

• Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
• Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
• No need to fragment DNA before sequencing.
• Amplifies ds-cDNA in 8 hours or less.
• Compatible with all next generation sequencing platforms except Pacific Bioscience.

Outras notas

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Informações legais

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable