NUC201

Nuclei Isolation Kit: Nuclei PURE Prep

sufficient for 15 nuclei preparations (~1-10×10⁷ cells or 1g of tissue per preparation)

• Sinônimo(s): Sucrose centrifugation nuclei isolation
• Código UNSPSC: 12352207
• NACRES: NA.32

Propriedades

• uso: sufficient for 15 nuclei preparations (~1-10×10⁷ cells or 1g of tissue per preparation)
• Nível de qualidade: 200
• embalagem: pkg of 1 kit
• condição de armazenamento: dry at room temperature
• aplicação(ões): cell analysis
• atividade externa: nuclease and protease, free
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C

Descrição

Aplicação

For preparation of pure nuclei and fragile nuclei from cell lines and solid tissues.

Ações bioquímicas/fisiológicas

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. The sucrose concentration that is suitable for a particular cell type is determined empirically by the user. The sucrose concentrate and sucrose cushion buffer give the user flexibility to modify the density of the sucrose cushion as appropriate. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Somente componentes do kit

• Nuclei PURE Lysis Buffer 180 mL

Informações de segurança

• Pictogramas: GHS05,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H315,H318,H410
• Declarações de precaução: P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Classificações de perigo: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable