NUC101

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)

• Sinônimo(s): Rapid mammalian nuclei isolation kit
• Código UNSPSC: 12352207
• NACRES: NA.32

Propriedades

• uso: sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)
• Nível de qualidade: 200
• prazo de validade: 1 yr at 2‑8 °C
• embalagem: pkg of 1 kit
• aplicação(ões): cell analysis
• atividade externa: nuclease and protease, free
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

Aplicação

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

Ações bioquímicas/fisiológicas

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Outras notas

All procedures should be carried out on ice or 2-8°C.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable