E7885

Elastase from porcine pancreas

lyophilized powder, suitable for cell culture

• Sinônimo(s): Elastase from hog pancreas, Pancreatopeptidase E
• CAS Number: 39445-21-1
• Número CE: 3.4.21.36 (BRENDA, IUBMB)
• Número CE: 254-453-6
• Número MDL: MFCD00130998
• Código UNSPSC: 12352204
• NACRES: NA.75

Propriedades

• fonte biológica: Porcine
• Nível de qualidade: 200
• Formulário: lyophilized powder
• atividade específica: ≥4 units/mg protein
• peso molecular: 25.9 kDa
• embalagem: pkg of 1 μmol
• técnica(s): cell culture | mammalian: suitable
• pH: 8
• adequação: suitable for
• atividade externa: Trypsin ≤50 BAEE units/mg protein
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

Aplicação

Elastase from porcine pancreas is used for the following applications:

• Cell culture techniques
• ELISA techniques
• Used in assay for elastase inhibitory activity

Ações bioquímicas/fisiológicas

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Embalagem

Package size based on protein content

Definição da unidade

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Informações de segurança

• Pictogramas: GHS08,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H315,H319,H334,H335
• Declarações de precaução: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable