PPB011

Tris Buffered Saline

powder, pH 7.6, pHast Pack^™

• Sinônimo(s): TBS
• Número MDL: MFCD00132476
• Código UNSPSC: 12161700
• NACRES: NA.25

Propriedades

• Product Name: Tris Buffered Saline, pH 7.6, pHast Pack^™, powder
• linha de produto: pHast Pack^™
• Nível de qualidade: 100
• Formulário: powder
• condição de armazenamento: (Tightly closed. Dry)
• Impurezas: ≤5 ppm Heavy Metals (lead)
• cor: white
• pH: 7.6
• solubilidade: soluble, clear, colorless
• traços de cátion: Fe: ~10 ppm
• atividade externa: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• temperatura de armazenamento: room temp

Descrição

Descrição geral

Tris Buffered Saline, or TBS, pH 7.6 is a commonly used wash buffer that is isotonic and non-toxic and ideal for use in cell culture and electrophoresis applications.

Aplicação

Tris-buffered saline can be used as a wash buffer for:

• Enzyme Linked Immunosorbent assay (ELISA)
• Western Blot and other protein applications
• Suitable for use in cell culture (recommend to sterile filter)
• Immunohistochemistry
• Sample preparation
• Resuspension and sonication of cells

Características e benefícios

• Ready for use, just dissolve in ultrapure water
• No calculations, measuring, and pH adjustments needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Embalagem

Foil pouches

Reconstituição

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl, pH 7.6 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture applications.

Armazenamento e estabilidade

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Outras notas

For additional information on our range of Biochemicals, please complete this form.

Informações legais

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1