PPB002

Tris Buffered Saline

powder, pH 8.0, pHast Pack^™, with 0.05% Tween^® 20

• Sinônimo(s): Tris Buffered Saline, with Tween™ 20, pH 8.0, TBS with 0.05% Tween-20, TBS-T, TBST
• Código UNSPSC: 41105323
• NACRES: NA.21

Propriedades

• Product Name: Tris Buffered Saline, with 0.05% Tween^® 20, pH 8.0, powder, pHast Pack^™
• linha de produto: pHast Pack^™
• Formulário: powder
• condição de armazenamento: (Tightly closed. Dry.)
• Impurezas: ≤5 ppm Heavy Metals (lead)
• cor: white
• pH: 7.8—8.2
• solubilidade: soluble, clear to hazy, colorless
• traços de cátion: Fe: ≤10 ppm
• atividade externa: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• temperatura de armazenamento: room temp

Descrição

Descrição geral

Tris Buffered Saline with 0.05% Tween^® 20 or TBST has a low concentration of detergent with a slightly basic pH of 8.0. It is a commonly used washing buffer alternative to PBS-T in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls.

Aplicação

Suitable for use as a wash buffer in:

• Western blot
• ELISA
• Immunohistochemistry

Características e benefícios

• Ready-to-use buffer requires no measurments or pH adjustments
• Saves time, money and effort to get your assays started fast
• Physical, chemical, and biological tests for quality: free of DNase, RNase, Protease, and Nickase
• Qualified for use in gel electrophoreis and other molecular biology assays

Embalagem

Foil pouches

Reconstituição

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Armazenamento e estabilidade

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Outras notas

For additional information on our range of Biochemicals, please complete this form.

Informações legais

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable