10831

Histopaque^®-1083

sterile-filtered, density: 1.083

• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• Quality Level: 400
• form: liquid
• shelf life: Expiry date on the label.
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• density: 1.083
• application(s): hematology; histology
• storage temp.: 2-8°C

Description

General description

Histopaque^®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a relative density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.

Application

Histopaque^®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.

Features and Benefits

Histopaque^®-1083 offers numerous advantages, such as:

• Optimal recovery of viable cells
• Permits selective separation of blood cell types
• Prevents cell distortion
• Preserves cell viability
• Minimal extraneous cell interference
• Reproducible performance from batch to batch
• Sterile-filtered and endotoxin-tested
• Stable at 2-8°C for at least 2 years when protected from light

Principle

Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment; whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290,H315,H319
• Precautionary Statements: P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves