M8537

MCDB 131 Medium

With ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): 131 Medium, Medium 131, Molecular, Cellular, and Development Biology 131 medium
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Product Name: MCDB 131 Medium, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
• form: powder
• concentration: 11.6 g/L
• technique(s): cell culture | mammalian: suitable
• components: sodium pyruvate: 0.11 g/L; phenol red: 0.012 g/L; L-glutamine: 1.461 g/L; HEPES: no; glucose: 1.0 g/L (Dextro)
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). 

Application

MCDB-131 Medium has been used:

• as a component of the basal medium to culture bovine retinal endothelial cells
• to culture bovine lung microvascular endothelial cells
• for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells

Quantity

Formulated to contain 11.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.18 g/L sodium bicarbonate.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable