73881

Fluo 3-AM

≥90% (TLC)

• Synonym(s): 4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4′-methyl-2,2′(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester
• Empirical Formula (Hill Notation): C₅₁H₅₀Cl₂N₂O₂₃
• CAS Number: 121714-22-5
• Molecular Weight: 1129.85
• MDL number: MFCD28143734
• UNSPSC Code: 12171500
• NACRES: NA.32

Properties

• Quality Level: 100
• Assay: ≥90% (TLC)
• form: powder
• fluorescence: λ_ex 506 nm; λ_em 525 nm in 0.1 M Tris pH 8.0, esterase
• storage temp.: −20°C
• SMILES string: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc(ccc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(OCOC(C)=O)c(Cl)cc35
• InChI: 1S/C51H50Cl2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2
• InChI key: PTPUOMXKXCCSEN-UHFFFAOYSA-N

Description

Application

Cell permeable fluorescent indicator of intracellular Ca²⁺; non-fluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca²⁺; lower binding affinity allows measurement of higher peaks of Ca²⁺ transients than with Fura 2.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable