14420C

EX-CELL^® 420 Serum-Free Medium for Insect Cells

with L-glutamine, sterile-filtered, suitable for insect cell culture

• MDL number: MFCD01634638
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• description: for research or for further manufacturing use
• Quality Level: 500
• sterility: sterile-filtered
• product line: EX-CELL^®
• form: liquid
• technique(s): cell culture | insect: suitable
• components: HEPES: no; L-glutamine: yes; glucose: yes; NaHCO₃: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or serum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 10⁷ cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.

EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or seum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 10⁷ cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable