1.52258

Chromolith^® WP 300 Protein A (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

• Synonym(s): Chromolith^® WP 300 Protein A 25-4.6 HPLC column
• UNSPSC Code: 41115700
• NACRES: SB.52

Properties

• Product Name: Chromolith^® WP 300 Protein A, L × I.D. 25 mm x 4.6 mm HPLC column
• material: PEEK column
• product line: Chromolith^®
• feature: endcapped: no; endcapped: no
• packaging: 1 ea of
• parameter: 2 - 45 °C temp. range; 2-45 °C temp. range; 200 bar pressure; 200 bar pressure
• technique(s): HPLC: suitable; HPLC: suitable; LC/MS: suitable
• column L × I.D.: 2.5 cm × 4.6 mm
• surface area: 120 m²/g
• matrix: monolithic silica; monolithic silica
• matrix active group: Protein A bonding phase; Protein A bonding phase
• pore size: 2 μm macroporosity; 2 μm macroporosity; 30 nm mesoporosity; 30 nm mesoporosity
• operating pH range: 1.5-7.5
• operating pH: 1.5-7.5
• separation technique: affinity
• storage temp.: 2-8°C; 2-8°C

Description

General description

Affinity chromatography is a selective technique which takes advantage of specific molecular interactions, for example antigen and antibody. The Chromolith^® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith^® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

Chromolith^® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith^® WP 300 Protein A HPLC columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany