ZRB2093

Anti-S100A1 Antibody, clone 1D18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: Clone, 1D18
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 10.55 kDa; observed mol wt ~9 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): mouse
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_006262
• UniProt accession no.: P23297
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... S100A1(6271)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D18 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects S100A1 protein. It targets an epitope within 13 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal half of human S100A1 protein.

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Human heart tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected S100A1 in Human heart tissue sections.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected S100A1 in lysate from Human heart tissue.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected S100A1 in Human heart tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound S100A1 peptide with a KD of 5.7 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected S100A1 in HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Protein S100-A1 (UniProt: P23297; also known as S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1) is encoded by the S100A1 (also known as S100A) gene (Gene ID: 6271) in human. S100A1 is a small calcium binding protein that plays important roles in several biological processes, including Ca2+ homeostasis, chondrocyte biology, and cardiomyocyte regulation. Typically, it exists as a homodimer, but it can also associate with two β-chains or as one - and one β-chain. It is highly expressed in the heart and serves as an important regulator of the cardiac contractility. In lesser quantities it is also found in skeletal muscle and brain. It is reported to regulate a network in cardiomyocytes controlling sarcoplasmic reticulum Ca2+ cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca2+-ATPase/ATP2A2 and mitochondrial F1-ATPase. It facilitates diastolic Ca2+ dissociation and myofilament mechanics in order to improve relaxation during diastole. S100A1 can also bind zinc in vitro, however, its binding sites are different from the calcium binding sites. Post-translational glutathionylation is shown to increase its affinity for calcium by about 10-fold. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Most, P., et al. (2003). J. Biol. Chem. 278(48); 48404-48412; Kiewitz, R., et al. (2003). Biochem. Biophys. Res. Commun. 306(2); 550-557; Most, P., et al. (2001). Proc. Natl. Acad. Sci. USA. 98(24); 13889-13894).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable