ZRB1365
Anti-Synaptophysin Antibody, clone 2B21, ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Major synaptic vesicle protein p38
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2B21, monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 33.31 kDa
observed mol wt ~34 kDa
species reactivity
rat, human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... SYP(6855)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 2B21 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Synaptophysin. It targets an epitope within 86 amino acids from the C-terminal region. It does not react with human Synaptophysin.
Immunogen
GST/His-tagged recombinant fragment corresponding to 86 amino acids from the C-terminal region of rat Synaptophysin.
Application
Anti-Synaptophysin, clone 2B21 ZooMAb, Cat. No. ZRB1365, a recombinant Rabbit monoclonal antibody that targets Synaptophysin and is tested in Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Synaptophysin in rat brain tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Synaptophysin in rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Synaptophysin in PC12 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Synaptophysin in rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Synaptophysin in PC12 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Synaptophysin (UniProt: P07825; also known as Major synaptic vesicle protein p38) is encoded by the Syp gene (Gene ID: 24804) in rat. Synaptophysin belongs to the synaptophysin/synaptobrevin family of proteins. It is one of the major glycoproteins present in presynaptic vesicles and in various neuroendocrine cells of both neuronal and epithelial phenotype. Its structure consists of a hexameric protein of four transmembrane spans with a short cytoplasmic amino- and a long carboxy-terminal tail. Both amino- and carboxy-terminals are exposed on the cytoplasmic surface of the synaptic vesicle membrane. The C-terminal domain of Synaptophysin is required for endocytosis during, but not after, cessation of sustained synaptic transmission. Synaptophysin is involved in organizing membrane components and may regulate endocytosis to ensure vesicle availability following sustained neuronal activity. It is also involved in the regulation of short-term and long-term synaptic plasticity. Syp/neurons display functional deficiencies and slower recovery of the recycling synaptic vesicle pool. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kwon, SE., and Chapman, ER (2011). Neuron. 70(5); 847- 854).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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Mian Wang et al.
Nature communications, 13(1), 3317-3317 (2022-06-11)
Digital light processing bioprinting favors biofabrication of tissues with improved structural complexity. However, soft-tissue fabrication with this method remains a challenge to balance the physical performances of the bioinks for high-fidelity bioprinting and suitable microenvironments for the encapsulated cells to
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