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Merck

T2391

Sigma-Aldrich

Triosephosphate Isomerase from rabbit muscle

Type III-S, ammonium sulfate suspension, ≥4,000 units/mg protein

Synonym(s):

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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About This Item

CAS Number:
9023-78-3
EC Number:
5.3.1.1 (BRENDA, IUBMB)
MDL number:
MFCD00082080
UNSPSC Code:
12352204
NACRES:
NA.54

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biological source

rabbit muscle

Quality Level

200

type

Type III-S

form

ammonium sulfate suspension

specific activity

≥4,000 units/mg protein

mol wt

calculated mol wt 53.2 kDa

foreign activity

3-phosphoglyceric phosphokinase ≤0.01%
glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
pyruvate kinase, lactic dehydrogenase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase and aldolase ≤0.001%

storage temp.

2-8°C

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Application

Triosephosphate isomerase has been used in a study to assess molecular characterizations of Cryptosporidium, Giardia, and Enterocytozoon. Triosephosphate isomerase has also been used in a study to investigate apoptosis of hepatocellular carcinoma cell lines.

Unit Definition

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCF6065
SLBW0197
SLBR7244V
SLBB4705V
020M7430

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