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Product Name
RENE 1, 92092510
biological source
rat endometrium
packaging
tube of 5 μg 92092510-DNA-5UG
pkg of vial of cells 92092510-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Cuboidal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Sprague-Dawley, primary endometrial, adenovirus transformed
Cell Line Description
Derived from the endometrium tissue from the uterus of a 21-28 day old Sprague-Dawley rat. Cells carry vimentin, connexin 26 and connexin 43 markers. The transforming agent is adenovirus E1a protein.
Culture Medium
DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 48 hours.
Other Notes
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