ANGM-CSS

8040401, human brain, Fusiform, can form heaps of cells

• UNSPSC Code: 41106514

Properties

• Product Name: ANGM-CSS, 08040401
• biological source: human brain
• packaging: tube of 5 μg 08040401-DNA-5UG; pkg of vial of cells 08040401-1VL
• growth mode: Not specified
• karyotype: Near tetraploid; range 88 to 91 - see Cell Line Description for more information
• morphology: Fusiform, can form heaps of cells
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human glioblastoma

Cell Line Description

A human A human glioblastoma multiforme cell line derived from a 65-year-old male. The results of immunocytochemical analysis for GFAP, Vimentin, NestiN and CD33 were positive in early and late passage cells. MGMT methylation was negative. Karyotype: Near tetraploid; range 88 to 91 with two copies of marker: der(6;7;12;)del(6q ), t(6;11), t(6;17) del(17q). Six copies of chromosome 7 and other rearrangements with loss and gain of chromosome fragments involving chromosomes 1,2,3,5,10,9,19,20. The X and Y chromosomes are present. (Information provided by Depositor)

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂;37ºC.

Other Notes

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