KARPAS 1106P

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human blood (peripheral)
• packaging: tube of 5 μg 06072607-DNA-5UG; pkg of vial of cells 06072607-1VL
• growth mode: Aggregates in suspension
• karyotype: Refer to Nacheva et al., 1994
• morphology: Lymphoblastoid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human B-Cell Non-Hodgkin′s Lymphoma

Cell Line Description

A human B cell lymphoma established from a pleural effusion of a 23-year-old woman with mediastinal lymphoblastic B-cell non-Hodgkin′s lymphoma. This cell line does not express BCL2.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10 - 20% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 0.5 - 1.5 x1000,000 cells/ml; 5% CO₂; 37°C. Doubling time approximately 60-100 hours. Split saturated culture 1:2 to 1:3 every 2-3 days. Spherical cells growing in clusters in suspension. This cell line can be grown with 10% Foetal Bovine Serum rather than 20%.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.