A3574

Acrylamide/Bis-acrylamide solution

30%, BioReagent, suitable for electrophoresis, 29:1

• Synonym(s): 30% Acrylamide/Bisacrylamide 29:1, Polyacrylamide solution
• MDL number: MFCD00080848
• UNSPSC Code: 41105319
• PubChem Substance ID: 329770837
• NACRES: NA.25

Properties

• Product Name: Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 29:1
• sterility: 0.2 μm filtered
• Quality Level: 200
• product line: BioReagent
• form: liquid
• feed ratio: 29:1
• technique(s): electrophoresis: suitable
• suitability: suitable for electrophoresis
• foreign activity: Protease, none detected
• storage temp.: 2-8°C; 2-8°C

Description

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent.[1] On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.[2]

Application

Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 4% stacking gel and 10% resolving gel during sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis.[3] It has also been used as a precursor for polyacrylamide lid fabrication by photopatterning methods.[4]

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H302 + H332,H315,H317,H319,H340,H350,H361fd,H372
• Precautionary Statements: P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Target Organs: Peripheral nervous system
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves