PS1008-M

PhosphoDetect Anti-FAK (pTyr⁸⁶¹) Rabbit pAb

liquid, Calbiochem^®

• Synonym(s): Anti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 100
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: mouse, human, chicken
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pTyr861)
• Gene Information: human ... PTK2(5747)

Description

General description

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic phosphotyrosine-containing peptide, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated at Tyr⁸⁶¹.

Recognizes the ~125 kDa FAK protein phosphorylated at Tyr⁸⁶¹ in primary chick embryo fibroblasts transfected with human FAK.

This PhosphoDetect Anti-FAK (pTyr⁸⁶¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of FAK (pTyr⁸⁶¹).

Immunogen

a synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK

Warning

Toxicity: Irritant (B)

Physical form

In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).

Analysis Note

Positive Control
Chicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin

Other Notes

Eliceiri, B.P., et al. 2002. J. Cell. Biol.157, 149.
Hauck, C.R., et al. 2002. J. Biol. Chem.277, 12487.
Lacalle, R.A., et al. 2002. J. Cell. Biol.157, 277.
Lim, Y., et al. 2002. J. Biol. Chem.277, 12735.
Melendez, J., et al. 2002. J. Biol. Chem.227, 45203.
Rigacci, S., et al. 2002. J. Biol. Chem.277, 41631.
Datta, A., et al. 2001. Mol. Cell. Biol.21, 7295.
Nakamura, K., et al. 2001. Oncogene20, 2626.
Rocic, P., et al. 2001. J. Biol. Chem.276, 21902.
Slack, J.K., et al. 2001. Oncogene20, 1152.
Sieg, D.J., et al. 2000. Nature Cell. Biol.2, 249.
Vial, D., et al. 2000. J. Biol. Chem.275, 28269.
Wennerberg, K., et al 2000. Mol. Cell. Biol.20, 5758.

The immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1