AB16941
Anti-Apaf-1 Antibody, NT
Chemicon®, from rabbit
Synonym(s):
Apoptosis Protease-activating Factor 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... APAF1(317)
Specificity
Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and was designated Apaf1 for apoptosis protease-activating factor 1(Zou et al. 1997; Cecconi et al. 1998). Apaf1 binds to cytochrome C (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 which is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (Li et al. 1997). Apaf1 can also associate with caspase-4 and caspase-8 (Hu et al. 1998). Apaf1 is ubiquitously expressed in human tissues (Zou et al. 1997).
Immunogen
Epitope: N-terminus
Polyclonal antibody raised against a peptide corresponding to amino acids 12 to 28 of human Apaf1 (Zou et al. 1997) The sequences of the immunogenic peptide are identical between human and mouse (Zou et al. 1997; Cecconi et al. 1998).
Application
Anti-Apaf-1 Antibody, N-terminus is an antibody against Apaf-1 for use in WB.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western blot: 1:1000 to 1:4000
Whole cell lysate from HeLa cells can be used as a positive control and a 130 kDa band should be detected. Optimal working dilutions must be determined by end user.
Whole cell lysate from HeLa cells can be used as a positive control and a 130 kDa band should be detected. Optimal working dilutions must be determined by end user.
Linkage
Replaces: 04-579
Physical form
Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
Format: Purified
Storage and Stability
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Carla L O'Connor et al.
Biochimica et biophysica acta, 1783(10), 1903-1913 (2008-07-02)
X-linked-inhibitor-of-apoptosis-protein (XIAP) is the most potent intracellular inhibitor of caspases-9, -3 and -7. While highly elevated XIAP levels reduce the apoptotic response to various stimuli, the potency of physiological XIAP expression to control caspase activation and the consequences of XIAP
Tumor necrosis factor death receptor signaling cascade is required for amyloid-beta protein-induced neuron death.
Li, R; Yang, L; Lindholm, K; Konishi, Y; Yue, X; Hampel, H; Zhang, D; Shen, Y
The Journal of Neuroscience null
M Cozzolino et al.
Cell death and differentiation, 11(11), 1179-1191 (2004-07-17)
Deficiency of the apoptosome component Apaf1 leads to accumulation of supernumerary brain cells in mouse embryos. We observed that neural precursor cells (NPCs) in Apaf1(-/-) embryos escape programmed cell death, proliferate and retain their potential to differentiate. To evaluate the
Mary E Lucas et al.
The Journal of biological chemistry, 284(22), 14698-14709 (2009-03-28)
Epigenetic silencing involving the aberrant DNA methylation of promoter-associated CpG islands is one mechanism leading to the inactivation of tumor suppressor genes in human cancers. However, the molecular mechanisms underlying this event remains poorly understood. TMS1/ASC is a novel proapoptotic
Differential expression of apoptotic protease-activating factor-1 and caspase-3 genes and susceptibility to apoptosis during brain development and after traumatic brain injury.
A G Yakovlev, K Ota, G Wang, V Movsesyan, W L Bao, K Yoshihara, A I Faden
The Journal of Neuroscience null
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