70781
pET Expression System 30 - Novagen
pET vectors plus host strains with induction control
Synonym(s):
pET-30a-c(+) Vectors
About This Item
Recommended Products
conjugate
S-Tag
Quality Level
tag
His tagged
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
dry ice
wet ice
storage temp.
−70°C
Related Categories
1 of 4
This Item | 1.46761 | 1.46004 | 1.46703 |
|---|---|---|---|
| technique(s) microbiological culture: suitable | technique(s) cell counting: suitable (total viable count (TVC)) | technique(s) - | technique(s) - |
| form plate | form plate (ready-to-use) | form plate (ready-to-use) | form plate |
| application(s) bioburden testing | application(s) filtration | application(s) air monitoring, cosmetics | application(s) air monitoring |
| feature ready-to-use, ICR Settle plate | feature ready-to-use | feature ready-to-use, Settle Plate for long Incubation | feature lid ICR plus: lockable settle plates, ready-to-use |
| packaging box of 120 plates | packaging pkg of (55 mm plate (20 plates per pack)) | packaging pack of 120 plates, pack of 20 plates | packaging triple packed of 20 and 120 plates |
General description
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.
Components: pET Expression Systems Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
- 10 µg pET vector DNA (for each indicated plasmid)
- 0.2 ml BL21 Glycerol Stock
- ml BL21(DE3) Glycerol Stock
- 0.2 ml BL21(DE3)pLysS Glycerol Stock
- 0.2 ml Induction Control Glycerol Stock
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
- 0.2 ml NovaBlue Competent Cells
- 0.2 ml BL21(DE3) Competent Cells
- 0.2 ml BL21(DE3)pLysS Competent Cells
- 2 × 0.2 ml SOC Medium
- 10 µl Test Plasmid
Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.
pET Expression System 3
The pET Expression System 3 contains 10 µg each of the four versions of pET-30 (pET-30a–c(+)). The pET-30a–c(+) vectors carry an N-terminal HIS TAG®/thrombin/S•Tag/enterokinase configuration plus an optional C-terminal HIS TAG sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB095VM.
Components
- 10 µg - eachpET-30a-c(+) vector DNA
- 0.2 ml- Host bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
- 0.2 ml- Induction control clone, glycerol stock
Warning
Legal Information
Disclaimer
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Clickhere for Enduser Declaration (EUD) Form.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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