5.05855

Adenovirus Proteinase Inhibitor

powder, ≥90% (HPLC)

• Synonym(s): Adenovirus Proteinase Inhibitor, NSC 37249, 2-(Dicyclohexylamino)-N-(4-(4-((2-(dicyclohexylamino)acetyl)amino)phenyl)sulfonylphenyl)acetamide, N,Nʹ-(Sulfonyldi-4,1-phenylene)bis(2-(dicyclohexylamino)acetamide, AVP Inhibitor, AVP Inhibitor, 2-(Dicyclohexylamino)-N-(4-(4-((2-(dicyclohexylamino)acetyl)amino)phenyl)sulfonylphenyl)acetamide, N,Nʹ-(Sulfonyldi-4,1-phenylene)bis(2-(dicyclohexylamino)acetamide
• Empirical Formula (Hill Notation): C₄₀H₅₈N₄O₄S
• CAS Number: 2907-88-2
• Molecular Weight: 690.98
• MDL number: MFCD31800417
• UNSPSC Code: 12352202
• NACRES: NA.21

Properties

• Product Name: Adenovirus Proteinase Inhibitor, NSC 37249,
• Assay: ≥90% (HPLC)
• Quality Level: 100
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: off-white
• solubility: 5% TFA in ethanol: 5 mg/mL; DMSO: insoluble
• storage temp.: 2-8°C
• SMILES string: [S](=O)(=O)(c4ccc(cc4)NC(=O)CN(C6CCCCC6)C5CCCCC5)c1ccc(cc1)NC(=O)CN(C3CCCCC3)C2CCCCC2
• InChI: 1S/C40H58N4O4S/c45-39(29-43(33-13-5-1-6-14-33)34-15-7-2-8-16-34)41-31-21-25-37(26-22-31)49(47,48)38-27-23-32(24-28-38)42-40(46)30-44(35-17-9-3-10-18-35)36-19-11-4-12-20-36/h21-28,33-36H,1-20,29-30H2,(H,41,45)(H,42,46)
• InChI key: JQYJJPSTWWRURQ-UHFFFAOYSA-N

Description

General description

A sulfonyldiphenylene-bis-dicyclohexylaminoacetamide compound that acts as a potent and selective inhibitor against adenovirus AVP, the cysteine proteinase critical for virion assembly and maturation, by targeting simultaneously AVP co-factor pVIc (GVQSLKRRRCF) N-terminal binding pocket (K_i = 150 nM) and AVP-pVIc substrate-binding site (K_i = 400 nM), blocking not only AVP-pVIc active complex formation/AVP activation (IC₅₀ = 140 nM; [substrate] = 5 µM & [pVIc] = 40 µM), but also AVP-pVIc active complex catalytic activity (IC₅₀ = 490 nM; [substrate] = 5 µM). Does not affect the activity of the serine protease trypsin or the cysteine protease papain even at concentrations as high as 10 µM.

Biochem/physiol Actions

Primary Target
AVP co-factor pVIc (GVQSLKRRRCF) N-terminal binding pocket

Reversible: yes

Secondary Target
AVP-pVIc substrate-binding site

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

McGrath, W.J., et al. 2013. FEBS Lett.587, 2332.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable