07-450-S

Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size

Upstate^®, from rabbit

• Synonym(s): H3K9me1, Histone H3 (monomethyl K9), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, chicken, human
• manufacturer/tradename: Upstate^®
• technique(s): dot blot: suitable; immunocytochemistry: suitable; inhibition assay: suitable (peptide); western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_003493
• UniProt accession no.: Q16695
• shipped in: wet ice
• target post-translational modification: monomethylation (Lys9)
• Gene Information: human ... HIST1H3F(8968)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Broad species cross-reactivity is expected based on sequence homology.

Application

Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size is a purified rabbit polyclonal that detects Histone H3 monomethylated on lysine 9 (H3K9me1). This Ab is specificity verified by dot blot (DB), and validated in ICC, PIA, WB. The small pack size is ideal for screening applications.

Quality

Routinely evaluated by Western Blot on acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3.

Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).

Target description

17 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1